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2D HSQC-ROESY
DESCRIPTION
The 2D HSQC-ROESY experiment is an hybrid inverse experiment consisting of an initial HSQC pulse train followed by a ROESY mixing sequence. In the first step, magnetization is transferred from the X nucleus (13C or 15N) to the directly-bonded proton nuclei via 1J(CH). In the second step, polarization transfer is achieved in the transverse plane via NOE. Similar results are obtained from the analog 2D HMQC-ROESY experiment.

REQUIREMENTS
Easy implementation on any AVANCE spectrometer equipped with an inverse probehead.
VERSIONS
The basic sequence of the 2D HSQC-ROESY experiment is based in the 2D HSQC experiment in which a ROESY mixing sequence (for instance, low-power continuous wave) is inserted prior to acquisition ( 92JMR575-100 ). Carbon decoupling is optionally applied during proton acquisition.

Improved results can be obtained from the gradient enhanced version (see ge-2D HSQC-ROESY experiment).

EXPERIMENTAL DETAILS
The 2D HSQC-ROESY experiment can be recorded in routine/automation modes. The experiment is basically optimized as described for the 2D HSQC experiment. The most important paramter to optimize is the mixing time length.

More details on practical implementation of 2D HSQC-ROESY experiments on AVANCE spectrometers can be found in

  • Tutorials: 2D inverse experiments
  • Tutorials: 2D gradient-based inverse experiments
    • SPECTRA
    The HSQC-ROESY spectrum correlates chemical shifts of heteronucleus X (F1 dimension) and protons (F2 dimension) via the direct heteronuclear coupling 1J(XH). Two different cross-peaks are present for each X resonance: The direct 1H-X NOE connectivites and the NOE 1H-X connectivities due to the cumulative transfer 1J(XH) + 1H-1H NOE.  The same NOE effects could also be detected by 2D HMQC-NOESY, 2D HMQC-ROESY and 2D HSQC-NOESY experiments.
    RELATED TOPICS
    Comparison of different 2D inverse correlations have been carried out ( 90JMR488-87 ).

    A 3D HSQC-ROESY experiment is easily designed by incorporating a second variable evolution period between the HSQC and the ROESY mixing period. On the other hand, a very similar pulse sequence is used for the w1-X-filtered 2D ROESY experiment by fixing the variable period in the HSQC block of this 3D HSQC-ROESY experiment.

    Related experiments:

  • 2D Inverse experiments
  • 2D Inverse gradient-enhanced experiments