The 2D RELAY-NOESY experiment is an hybrid experiment consisting of consecutive RELAY and NOESY building blocks. In the first step, magnetization is transferred between protons via J scalar coupling and in the second step, such magnetization is propagated via NOE.

Easy implementation on AVANCE spectrometers.

The basic pulse sequence of the NOESY-Relayed experiment consists of a conventional 2D RELAY pulse sequence followed by a NOESY building block ( 84JMR497 and 88MRC919 ).

Modified experiments:

• A related RELAY-ROESY experiment could also been designed by replacing the NOESY block by a ROESY block.
• On the other hand, the two blocks can be reversed to obtain a 2D NOESY-RELAY experiment.
• Improved results can be obtained from a 2D TOCSY-NOESY experiment.

The 2D RELAY-NOESY experiment can be recorded in routine/automation modes. The most important parameter to consider is the length of the  the RELAY and NOESY mixing periods. See 2D RELAY and 2D NOESY experiments for more details.

Tutorials: 2D homonuclear experiments

Tutorials: 2D gradient-based homonuclear experiments

Tutorials: 2D gradient-based homonuclear experiments in H₂O

In the RCT-NOESY experiment, the two-step magnetization transfer from a spin 1 (in F1) via spin 2 to a spin 3 (in F2) requires a J-coupling between 1 and 2 and a NOE transfer between 2 and 3. However, the requeriments for a transfer from spin 3 (in F1) via spin 2 to a spin 1 are different. That means RCT-NOESY spectra are asymmetrical with respect to the diagonal. In addition, cross-peaks suffer from worse antiphase cancellation because of the low digital resolution in the F1 dimension (antiphase pattern in F1 and in-phase pattern in F2).  These disadvantages can be partially overcomed performing 2D TOCSY-NOESY or 2D TOCSY-ROESY experiments.

Related experiments:

2D homonuclear experiments

2D gradient-based homonuclear experiments