The 2D DQ-TOCSY experiment is an hybrid experiment consisting of consecutive DQ and TOCSY building blocks. In the first step, magnetization is transferred between J-coupled protons via  double-quantum coherences (DQCs) and in the second step, such magnetization is propagated via a TOCSY transfer. It allows to detect remote connectivities as described in TOCSY experiment, differing only in the different resonance line positions.

Easy implementation on AVANCE spectrometers.

The basic pulse sequence of the DQ-TOCSY experiment consists of a refocused 2D DQ pulse sequence followed by a TOCSY building block ( 89JMR631-81 ). A related 2D ZQ-TOCSY experiment ( 90JMR633-87 ) has been proposed by selecting ZQCs insteaf of DQCs during the first block.

The 2D DQ-TOCSY experiment can be recorded in routine/automation modes. The most important parameter to consider is the length of the DQ and TOCSY mixing periods. See 2D DQ and 2D TOCSY experiments for more details.

Tutorials: 2D homonuclear experiments

Tutorials: 2D gradient-based homonuclear experiments

Tutorials: 2D gradient-based homonuclear experiments in H₂O

2D DQ-TOCSY spectra will show direct connectivities as in conventional 2D DQ spectra as well as remote connectivities arising from the TOCSY transfer.

Related experiments:

2D homonuclear experiments

2D gradient-based homonuclear experiments