Tutorials: 2D 1H-13C Inverse&Gradient experiments

ge-2D ¹H-¹³C Inverse

Go to eNMR

Interesting Note: All described pulse programs use the f2 decoupler channel for the ¹³C nucleus. Thus, they can also be applied on other nuclei, for instance, if a two-channel broadband probehead is available. In such case, pulses, delays and gradient strengths should be set accordingly.

ge-2D HMQC experiment

Magnitude-mode ge-2D HMQC (hmqcgpqf | HMQCGP)

Phase-sensitive ge-2D HMQC using z-filter (hmqcgpph)
Phase-sensitive ge-2D HMQC using echo-antiecho (hmqcetgp)
Phase-sensitive ge-2D HMQC using echo-antiecho with adiabatic pulse for inversion and refocussing (hmqcetgp.2)
Phase-sensitive ge-2D HMQC using PEP (hmqcetgpsi)
Phase-sensitive ge-2D HMQC using PEP and shorter overall timing (hmqcetgpsi.2)

ge-2D HSQC experiment

Phase-sensitive ge-2D HSQC using z-filter and selection before t1 (hsqcgpph)
Phase-sensitive ge-2D HSQC using z-filter and selection after t1 (hsqcgpph2)
Phase-sensitive ge-2D HSQC using echo-antiecho (hsqcetgp | HSQCETGP)
Phase-sensitive ge-2D HSQC using echo-antiecho and adiabatic pulses for inversion (hsqcetgpsp)
Phase-sensitive ge-2D HSQC using echo-antiecho and adiabatic pulses for inversion and refocusing(hsqcetgpsp.2)
Phase-sensitive ge-2D HSQC using echo-antiecho and adiabatic pulses for inversion and refocusing and BS effects(hsqcetgpsp.3)
Phase-sensitive ge-2D HSQC using PEP (hsqcetgpsi)
Phase-sensitive ge-2D HSQC using PEP with gradients in back-inept (hsqcetgpsi2) | HSQCGP)
Phase-sensitive ge-2D HSQC using PEP and adiabatic pulses for inversion(hsqcetgpsisp | HSQCETGPSISP)
Phase-sensitive ge-2D HSQC using PEP and adiabatic pulses for inversion with gradients in back-inept (hsqcetgpsisp2)
Phase-sensitive ge-2D HSQC using PEP and adiabatic pulses for inversion and refocusing (hsqcetgpsisp.2 | HSQCETGPSISP.2)
Phase-sensitive ge-2D HSQC using PEP and adiabatic pulses for inversion and refocusing with gradients in back-inept (hsqcetgpsisp2.2)
Phase-sensitive ge-2D HSQC using PEP and adiabatic pulses for inversion and refocusing with gradients in back-inept and presaturation (hsqcetgpprsisp2.2)
Phase-sensitive ge-2D HSQC using PEP and adiabatic pulses for inversion and refocusing with gradients in back-inept and suppression of COSY-type artefacts (hsqcetgpsisp2.3)
Phase-sensitive ge-2D HSQC using echo-antiecho and WET (hsqcetgpsiwt | HSQCETGPSIWT)
Phase-sensitive ge-2D HSQC using water flip-back pulses and PEP with gradients in back-INEPT (hsqcetfpgpsi2)
ge-2D HSQC-IPAP experiment

ge-2D ¹H-¹³C HSQC-IPAP (hsqcetgpiasp)

ge-2D CT-HSQC experiment

Phase-sensitive Constant-Time ge-2D HSQC using adiabatic pulses (hsqcctetgpsp)
Phase-sensitive Constant-Time ge-2D HSQC using adiabatic pulses and without CO refocussing (hsqcctetgpsp.2)
Phase-sensitive Constant-Time ge-2D HSQC using adiabatic pulses and PEP (hsqcctetgpsisp)
ge-2D CT-HMQC experiment

Phase-sensitive Constant-Time ge-2D HMQC using adiabatic pulses (hmqcctetgp)
Phase-sensitive Constant-Time ge-2D HMQC (hmqcctetgp.2)

ge-2D multiplicity-edited HSQC experiment

Phase-sensitive ge-2D multiplicity-edited HSQC using z-filter (hsqcedgpph)
Phase-sensitive ge-2D multiplicity-edited HSQC using echo-antiecho (hsqcedetgp | HSQCEDETGP)
Phase-sensitive ge-2D multiplicity-edited HSQC using echo-antiecho and adiabatic pulses (hsqcedetgpsp)
Phase-sensitive ge-2D multiplicity-edited HSQC using echo-antiecho and matched sweep adiabatic pulses (hsqcedetgpsp.3)
Phase-sensitive ge-2D multiplicity-edited HSQC using PEP and adiabatic inversion pulses (hsqcedetgpsisp)
Phase-sensitive ge-2D multiplicity-edited HSQC using PEP and adiabatic inversion pulses with gradients in back-inept (hsqcedetgpsisp2)
Phase-sensitive ge-2D multiplicity-edited HSQC using PEP and inversion and refocusing adiabatic pulses (hsqcedetgpsisp.2)
Phase-sensitive ge-2D multiplicity-edited HSQC using PEP and inversion and refocusing adiabatic pulses with gradients in back-inept (hsqcedetgpsisp2.2)
Phase-sensitive ge-2D multiplicity-edited HSQC using PEP and inversion, refocusing and matched sweep adiabatic pulses with gradients in back-inept (hsqcedetgpsisp2.3)
Phase-sensitive ge-2D multiplicity-edited HSQC using PEP and inversion, refocusing and matched sweep adiabatic pulses with gradients in back-inept and suppression of COSY-type artefacts (hsqcedetgpsisp2.4)

ge-2D TROSY experiment

Phase-sensitive ge-2D TROSY with presaturation (trosygpphpr)
ge-2D TROSY for aromatic residues with WATERGATE (trosyargpphwg)

ge-2D X-INEPT experiment

Phase-sensitive ge-2D X-INEPT using echo-antiecho (xhcoetgp)

ge-2D HMBC experiment

Magnitude-mode ge-2D HMBC (hmbcgpndqf | HMBCGPND)
Magnitude-mode ge-2D HMBC using low-pass J-filter (hmbcgplpndqf | HMBCGP)
Magnitude-mode ge-2D HMBC using low-pass J-filter and presaturation (hmbcgplpndprqf)
Magnitude-mode ge-2D HMBC using double low-pass J-filter (hmbcgpl2ndqf)
Phase-sensitive ge-2D HMBC using echo-antiecho(hmbcetgpnd)
Phase-sensitive ge-2D HMBC using a two-fold low-pass J-filter (hmbcetgpl2nd)
Phase-sensitive ge-2D HMBC using a three-fold low-pass J-filter (hmbcetgpl3nd)
Phase-sensitive ge-2D Multiplicity-edited HMBC using a three-fold low-pass J-filter (hmbcedetgpl3nd)
Magnitude-mode CIGAR-HMBC without decoupling (hmbcacgplpndqf)
Magnitude-mode CIGAR-HMBC without decoupling (hmbcacgplpndqf.2)
Magnitude-mode CIGAR-HMBC with decoupling (hmbcacgplpqf)
Magnitude-mode band-selective ge-2D HMBC without decoupling (shmbcgpndqf)
ge-2D HSMC (hmscetgpnd)
ge-2D 2J,3J HMBC, STAR-HMBC (hmbcacbigpl2ndqf)
Phase-sensitive ge-2D HAT-HMBC using a three-fold low-pass J-filter (hathmbcetgpl3)
ge-2D Constant-Time HMBC (CT-HMBC) using echo-antiecho (hmbcctetgpnd)
ge-2D Constant-Time HMBC (CT-HMBC) using echo-antiecho and low-pass J-filter (hmbcctetgpl2nd)

Long-range optimized ge-2D HSQC

Phase-sensitive ge-2D long-range optimized HSQC (HSQMBC) using adiabatic pulses (hsqcetgplrsp)
Phase-sensitive ge-2D long-range optimized HSQC (HSQMBC) using G-BIRD (hsqcetgpjclrnd)
ge-2D long-range optimized J-HSQC (EXSIDE) (hsqcetgplrjcsp)

ge-2D Long-range HCCH correlation

Phase-sensitive ge-2D Long-range HCCH using echo-antiecho (hcchetgplr)

);