Standard line shape test sample: 60% benzene/40% p-dioxane.

Insert the sample (ij).

Choose the solvent deuterium signal with the lock command

Tune and match the probehead (wobb or atma if required).

Optimize the shim procedure (read an optimized shim file with the rsh command or performs a gradshim if required).

Record a conventional ¹H spectrum.

Record a conventional ¹³C spectrum.

The calibration of the ¹³C line shape is performed using the standard C13HUMP parameter set (the pulse program is set to pulprog zgcw30). The width of the benzene resonance line is measured at the height of 0.55% and 0.11%. 

The test is usually performed using a sample spinning rate of 20 Hz. Update the corresponding pulses and power levels in the acquisition parameters according to the selected solvent/probehead parameters by executing the getprosol command (pulses and power levels must be correctly set by the edprosol command). If required, any acquisition parameter can be modified manually or in the AcquPars section.

o1 and o2 are set on resonance of the ¹³C and ¹H benzene resonances, respectively.

Set p1 to the 90º ¹³C observe pulse and pl14 to the low power level for ¹H decoupler pulse decoupler attenuation for continuous wave decoupling.

Start acquisition by rga and zg (the expected experimental time is displayed with the expt command).

The recorded data is Fourier transformed with ft (or ef) and phase and baseline corrections are performed using apk and abs, respectively. Modify processing parameters if required from the ProcPars section or using the ased command.. Reference the benzene line to 0.0 Hz.

Use the TOPSPIN plot editor (xwinplot) Plot from 7 to -8 Hz with a CX=15 cm (1.0 Hz/cm) and a CY=1000 cm. Determine the lineshape at 5.5 cm and 1.1 cm height. The plot window must also show the spinning sidebands.