Standard ³¹P sensitivity test sample: 0.0485M Triphenylphosphate (TPP) in acetone-d₆.

Record a ¹H-coupled ³¹P spectrum.

The calibration of the ³¹P sensitivity test is performed using the standard ¹H-coupled ³¹P parameter set (the pulse program is set to pulprog zg).  

It is very important to use a long recycle delay, usually d1=5*T₁(³¹P) (for instance d1=10 s). Use a single scan (ns=1).

Set p1 to the 90º ³¹P observe pulse at high power level pl1. This pulse is calibrated reaching the maximum signal for 90º and null point in 180º.

o1 is set on-resonance of the TPP resonance.

Transform the spectra using ef (lb=5 Hz) and adjust the phase to pure absorption. Reference the line to 0 Hz and plot the spectrum between 4000 and -4000 Hz. See spectrum

 Plot the noise with a minimum amplitude of 1.5 cm over a minimum of 1000 Hz.