Standard ¹⁵N sensitivity test sample: 90% formamide in DMSO-d₆.

Record a ¹H-decoupled ¹⁵N spectrum.

The calibration of the ¹⁵N sensitivity test is performed using the standard ¹H-decoupled ¹⁵N parameter set (the pulse program is set to pulprog zgig). This test is performed using the inverse gated decoupling scheme in order to avoid the build up of NOE. 

It is very important to use a long recycle delay, usually d1=5*T1(¹⁵N) (for instance d1=500 s). Use a single scan (ns=1).

Set p1 to the 90º ¹⁵N observe pulse at high power level pl1. This pulse is calibrated reaching the maximum signal for 90º and null point in 180º.

. Reference the line to 0 ppm and plot the full spectrum.

 Plot the noise with a minimum amplitude of 1.5 cm over a minimum of 100 Hz.