Sample: Cholesteryl Acetate
Cholesterol occurs widely in the human body and serve as an intermediate
in the biosynthesis of all of the steroids of the body.
Chemically, cholesterol (or 5-cholesten-3b-ol) is an steroid
containing the basic perhydrocyclopentanophenantrene
ring system. Thus, their 1H and 13C NMR spectra
consist of a
characteristic
overlapped aliphatic region which can be fully assigned
using 2D experiments.
A sample with 1g/ml Cholesterylacetate in CDCl3
(for 100-400MHz) or 1g/ml Cholesterylacetate in CDCl3
(for 500-800MHz) is used for the following tests: