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Once the CPMG experiment has been succesfully acquired, the following steps must be executed for a proper automated T1 calculation:
- From the 2D data set, move to a 1D data set containing the row for which vc is a maximum, for instance, by typing rser 1.
- The 1D data is transformed using ef (lb=1) and it is manually phase corrected and further stored as usual.
- Into the integrate subroutine, a separate region must be selected using the mouse for each peak for which T2 will be calculated. Click on return and select Save as 'intrng' and return. Enter wmisc to call up the menu of miscellaneous list types and click on intrng to select the range file type. Type the new name t2reg.
- Into the basl subroutine, select the points for which T2 will be calculated. One and only one point must be selected for each integral region defined above using the def-pts button. Take care to select the point of maximum intensity for each peak (region). Store the baseline points and enter wmisc to call up the menu of miscellaneous list types. Click on baslpnts to select the baseline point file type and type the new name t2bas.
- The 2D spectra is processed by the automation program proc_t1. Answer the questions as follows:
Enter fid no. for phase determination: 1
Enter left limit for baseline correction: 1000
Enter right limit for baseline correction: 1000
Enter no. of drift points: 20
Enter name of baseline point file: t2bas
Enter name of integral range file: t2reg
Enter name of VDlist to use: t2delay
Enter calc. type (T1=1, T2=2): 2
- View results in the t1/t2 subroutine. The first T2 curve should appear automatically in the window. Enter nxtp to view the T2 curves for successive peaks and use ct2 to begin the T2 calculation for each selected resonance.