Several standard parameter sets are delivered with TOPSPIN ready to use in automation mode. They are stored in the /<$TOPSPINHOME>/exp/stan/nmr/par/ directory and the basic protocol for their use is: Define the current probehead and pulse/power levels (edhead & edprosol) Read Standard Parameter Set (rpar) Set Solvent & Probehead properties (solvent & getprosol) Tune/Match (if required) Shimming (if required) Start Acquisition (rga and zg or alternatively using xaua) Start Processing (ft (for 1D) or xfb (for 2D) or alternatively using xaup)

Test and 1D Calibrations 1D 1H 1D 13C 1D inverse 1D Multinuclear 1D selective Solvent suppression 2D 2D 1H 2D 1H in H2O 2D 13C-detected 2D 1H-13C Inverse 2D 1H-15N Inverse Relaxation Others 2D & 3D Nucleic Acids X-filtered/edited 2D 3D LC-NMR STD Solid-State NMR Miscellaneous Backbone 3D Proteins Backbone Backbone & TROSY Backbone & 2H-decoupled Backbone & TROSY & 2H-decoupled Sidechain 3D Proteins Sidechain Sidechain & TROSY Sidechain & 2H-decoupled Sidechain & TROSY & 2H-decoupled Miscellaneous Proteins 3D sidechain 3D & 4D NOESY 2D & 3D Carbon-Detected Coupling Constants Aromatics Multimensional APSY BEST-NMR