• Insert the sample (ij).
• Choose the solvent deuterium signal with the lock command
• Tune and match the probehead (wobb or atma if required).
• Optimize the shim procedure (read an optimized shim file with the rsh command or performs a gradshim if required).
• Record a ¹H spectrum.

Create a new dataset (new) and read the standard BRUKER parameter set (rpar) to record a conventional 1D ¹H-¹³C HMQC spectra with rpar HMQC1D all and change the pulse program to iineptnd that can be visualized in the PulsProg section or with the edcpul command).

Update the corresponding pulses and power levels in the acquisition parameters according to the selected solvent/probehead parameters by executing the getprosol command (pulses and power levels must be correctly set by the edprosol command). If required, any acquisition parameter can be modified manually or in the AcquPars section.

d20 is the evolution delay d2 is optimized to 1/(6*¹J_CH) for all multiplicities.

On the other hand, the following standard acquisition parameters can be modified: d1, ns, ds, sw and td.

Start acquisition by rga and zg (the expected experimental time is displayed with the expt command).

The recorded data is Fourier transformed with ef (set lb=1) and automatic phase and baseline corrections are performed using apk and abs, respectively. Modify processing parameters if required from the ProcPars section or using the ased command.

Use the TOPSPIN plot editor (xwinplot)

It can be advisable to store all acquisition and processing parameters (with the command wpar) to be used later.

Both acquisition and processing steps can be performed using predefined automated AU programs. In this case, start data acquisition with the xaua command (the program executes the AU program defined in the acquisition parameters AUNM (au_zg)) and the data can be automatically processed and plotted with the xaup command (the program executes the AU program defined in the processing parameter AUNMP (proc_1d).

This experiment can also by recorded in full automation mode using macros (edmac), BUTTON-NMR (buttonnmr) or ICON-NMR (iconnmr)

See list of inverse 1D experiments