Select the NMR Experiment COSY COSY-DQF TOCSY NOESY ROESY ---------- HMQC HMQC-COSY HMQC-TOCSY HMQC-ROESY HMQC-NOESY DEPT-HMQC HSQC Multiplicity-edited HSQC HSQC-TOCSY HSQC-ROESY HSQC-NOESY TROSY HMBC ADEQUATE ---------- DEPT INEPT HETCOR INADEQUATE ---------- DOSY STD CLEANEX ---------- HNCO HNCA intra-HNCA Sequential-HNCA HNCOCA HNCACO HNCANH HNCAHA HCACO HCAN HCACON ---------- CBCACONH CBCANH Sequential-CBCANH HNCOCACB HNCACB intra-HNCACB Sequential-HNCACB HBHACONH HBHANH CCCONH CCACONH CCANH HCCCONH HCBCACON ---------- HCCH-COSY HCCH-TOCSY TOCSY-HSQC NOESY-HSQC HSQC-NOESY-HSQC X-filtered/edited NOESY ---------- HCN HCP Molecule under study: Small-to-medium sized natural-abundance Unlabeled Protein | Unlabeled Nucleic Acid 15N-Labeled Protein 13C,15N-Labeled Protein 2H,13C,15N-Labeled Protein Labeled Protein/Unlabeled Ligand Complex Labeled Nucleic Acid Dimensionality: 1D 2D 3D 4D Use of Gradients?: No Yes Data Representation: Magnitude-mode Phase-sensitive Solvent Supression?:No Yes Presaturation WATERGATE Excitation Scultping WET Water flip-back LC-NMR Inverse Spectroscopy Options: Using f2 channel Using f3 channel PEP TROSY 2H-decoupling Adiabatic Pulses Constant-Time Relaxation Options: NOE T1 T2 T1rho R(exchange) Cross-Correlation Coupling Constants Options: J-measurement in proteins Residual Dipolar Couplings Long-Range J(CH) IPAP E.COSY Quantitative-J Spin-echo difference Isotope Editing/Filtering Options in NOESY/TOCSY :No Yes: F1 F2 F1/F2 Doubly-13C,15N-filtering Simultaneous X,Y evolution Protein NMR Options: Carbon-Detected SOFAST-BEST methods Reduced Dimensionality (APSY) Amino-acid Selective (MUSIC)