a gradient-based 3D TOCSY-HSQC experiment with (the pulse program mlevhsqcetf3gp3d

Channel	f1	f3
Nucleus	1H	15N
Offset	o1p	o3p
Value	4.75	115.5

 

The following pulses applied on the three channels must be previously calibrated:

• Hard 90º and 180º ¹H pulses via the observe f1 channel (p1 and p2 at pl1).
• Low-power 90º ¹H pulse for broadband MLEV-17 isotropic mixing via the observe f1 channel
(p6 at pl10).
• Hard 90º and 180º ¹⁵N (or ¹³C) pulses via the decoupler f3 channel (p21 and p22 at pl3).
• 90º ¹⁵N (or ¹³C)  pulse for GARP decoupling during ¹H acquisition via the decoupler f3 channel. (pcpd3 at pl16 using cpdprg3=garp).

Otherwhise, all required acquisition parameters can be displayed with the ased command. By default:
 

 

Dimension	F3	F2	F1
Nucleus	1H	15N	1H
TD	2048	40	128
Spectral width	1H  (14 ppm)	N  (32 ppm)	1H (14 ppm)

 

Interpulse delays:

• d26 is optimized to 1/(4*cnst4) where cnst4=J_X-H (90 Hz).
• d9 is the TOCSY mixing time (55 ms)

All other delays are automatically calculated. Gradient parameters are already defined and the user must be set the number of scans and dummy scans (by default, ns=8 and ds=16) as a function of sample concentration.

The standard processing parameters are set to:
 

Dimension	F3	F2	F1
SI	2048	128	512
MC2	-	echo/antiecho	States-TPPI
WDW	QSINE	QSINE	QSINE
Offset	2	2	2
ME_mod	no	LPfc	no
NCOEF	0	32	0

 

List of Related Experiments:

Double-Resonance ¹H-¹³C experiments
Double-Resonance ¹H-¹⁵N experiments
Triple-Resonance ¹H-¹³C-¹⁵N backbone experiments
Triple-Resonance ¹H-¹³C-¹⁵N sidechain experiments