For 2H-labeled proteins, it is necessary to use a slighlty modifed 3D HN(CO)CA experiment. a gradient-based sensitivity-enhanced 3D HN(CO)CA experiment with 2H-decoupling by typing rpar HNCOCAGP2H3D all The pulse program hncocagp2h3d The major modifications, with respect to the original experiment, are the following: