3D HNCA
PRELIMINARY SET-UP PARAMETER SET
a gradient-based sensitivity-enhanced 3D HNCA experiment by typing (the hncagp3d pulse program
Channel
f1
f2
f3
Nucleus
1H
13C
15N
Offset
o1p
o2p
o3p
Carrier
4.75 ppm
51.67 ppm
115.5 ppm
MODIFY SPECIFIC PARAMETERS
The following hard and shaped pulses applied on the three channels must be previously calibrated:

    • Otherwhise, all required acquisition parameters can be displayed with the ased command. By default:
     

     
    Dimension
    F3
    F2
    F1
    Nucleus
    1H
    15N
    13C
    TD
    2048
    40
    128
    Spectral width
    NH 
    (14 ppm)
    N 
    (32 ppm)
    13CA
    (32 ppm)
     

    The following interpulse delays are defined by default:

                d21 is optimized to 1/(2*JN-H) (5.5 ms)
                d23 is optimized to 1/(4*JN-CA) (12 ms)
                d26 is optimized to 1/(4*JN-H) (2.3 ms)

    All other delays are automatically calculated from these values. The user must be set the and as a function of sample concentration.

    This experiment contains 3 gradients and the standard parameters are already defined with p16 of 1ms, d16=100u, gpnam1-3=SINE.100 and the ratio is 30:80:8.1

    ACQUISITION
    PROCESSING
    The standard processing parameters are set to:
     
    Dimension
    F3
    F2
    F1
    SI
    2048
    256
    512
    MC2
    -
    echo/antiecho
    States-TPPI
    WDW
    QSINE
    QSINE
    QSINE
    Offset
    2
    2
    2
     ME_mod
    no
    LPfc
    LPfc
    NCOEF
    0
    32
    32
    PLOT
    OBSERVATIONS

    OTHER RELATED VERSIONS
    List of available 3D HNCA versions.

    Any version of the 3D HNCA experiment can be automatically recorded as a 2D experiment. The only thing to do is to set the number of experiments (TD) in one of the two indirect dimensions to 1 (eda window). After start acquisition with zg, data can be processed from the 3D menu display using xfb (the number of procno must be typed). In this way, the 2D H(N)CA and the 2D HN(CA) experiments can be recorded under the same experimental conditions. In fact, these are the two first 2D planes of the 3D data.

    Related 3D Experiments.