a sensitivity-enhanced 3D HCCH-COSY experiment with (the pulse program hcchcogp3d

Channel	f1	f2	f3
Nucleus	1H	13C	15N
Offset	o1p	o2p	o3p
Value	4.75 ppm	38 ppm	115.5 ppm

 

. The following hard and shaped pulses applied on the three channels must be previously calibrated:

• Hard 90º and 180º ¹H pulses via the observe f1 channel (p1 and p2 at pl1).
• Hard 90º and 180º ¹³C pulses via the decoupler f2 channel (p3 and p4 at pl2).
• Selective 180º ¹³C pulse applied off resonance on the CO resonances via the decoupler f2 channel (p14 at sp5, using spnam5=G3.256, spoff5=135, and p14 about 256u).
• 90º ¹³C pulse for GARP ¹³C decoupling during ¹H acquisition via the decoupler f2 channel (pcpd2 at pl12 using cpdprg2=garp).
• Hard 180º ¹⁵N pulses via the decoupler f3 channel (p22 at pl3).

Otherwhise, all required acquisition parameters can be displayed with the ased command. By default:
 

 

Dimension	F3	F2	F1
Nucleus	1H	13C	1H
TD	2048	64	128
Spectral width	CH  (14 ppm)	13Caliphatic  (75 ppm)	CH  (14 ppm)

 

Interpulse delays:

            d4 is optimized to 1/(4*J_C-H) (1.8 ms)
            d21 is optimized to 1/(6*J_C-H) (1.1 ms)
            d24 is optimized to 1/(4*J_C-C) (3.6 ms)

All other delays are automatically calculated from these values. Gradient parameters are already defined and the user must be set the number of scans and dummy scans (ns=4 and ds=8) as a function of sample concentration.

The standard processing parameters are set to:
 

Dimension	F3	F2	F1
SI	2048	256	512
MC2	-	States-TPPI	States-TPPI
WDW	QSINE	QSINE	QSINE
Offset	2	2	2
ME_mod	no	LPfc	no
NCOEF	0	32	0

 

List of Related Experiments:

Double-Resonance ¹H-¹³C experiments
Double-Resonance ¹H-¹⁵N experiments
Triple-Resonance ¹H-¹³C-¹⁵N backbone experiments
Triple-Resonance ¹H-¹³C-¹⁵N sidechain experiments