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A phase-sensitive ge-2D NOESY experiment incorporating a water flip-back pulse during the mixing time and the WATERGATE scheme, load the parameter set defined for the ge-2D NOESY experiment using WATERGATE, by typing and getprosol, and change the pulse program to noesyfpgpph19.The only different parameters to be modified are: Perform data acquisition and data processing as described in the original experiment.
- The water-selective 90 pulse applied during the mixing time must be specified by p11 applied at a sp1 power level and with a spnam1 shape. phcor3 is the phase difference between this shaped pulse and a hard pulse.
- Gradients must be optimized to gpz1=50 (purgue gradient during the mixing time) and gpz2=30 (WATERGATE gradients).