Record a conventional 1H spectrum. Note the o1 of the selected resonance.

Record a SPFGE experiment on the selected ¹H resonance to be selectively excited. Check for the proper values of p12 and sp2.

a Selective gradient-enhanced 1D NOESY spectrum with rpar SELNOGP all (the pulse program selnogp

A minimum two-scan cycle is needed (ns=2 and ds=2).

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The best option is to use the shorter selective 180º pulse that achieve clean excitation of the selected resonance without disturb other resonances. In some cases, the use of long selective pulses can undergo important sensitivity losses due to relaxation effects.

The selective ge-1D NOESY experiment can also be recorded using a DPFGE scheme as a  starting building block (see DPFGE experiment for more details). NOE measurements can also be performed using the selective 1D ROESY analog. See details how to build-up a NOE curve as a function of the mixing time.