Insert the sample (ij).

Choose the solvent deuterium signal with the lock command

Tune and match the probehead (wobb or atma if required).

Optimize the shim procedure (read an optimized shim file with the rsh command or performs a gradshim if required).

Record a conventional 1H spectrum. Note the o1 of the selected resonance.

Record a SPFGE experiment on the selected ¹H resonance to be selectively excited. Check for the proper values of p12 and sp2.

Create a new dataset (new) and read the standard BRUKER parameter set (rpar) to record a Selective gradient-enhanced 1D NOESY spectrum with rpar SELNOGP all (the pulse program selnogp can be visualized in the PulsProg section or with the edcpul command).

Update the corresponding pulses and power levels in the acquisition parameters according to the selected solvent/probehead parameters by executing the getprosol command (pulses and power levels must be correctly set by the edprosol command). If required, any acquisition parameter can be modified manually or in the AcquPars section.

The most relevant parameters for an optimum selective excitation using SPFGE are:

p12 is the duration of the selective 180 pulse

sp2 is the required power level for a given shape defined in spnam2

spoffs2 is the offset (in Hz) to be selectively excited with respect to the center of the spectrum (o1)

The duration of the NOESY mixing time is defined by d8 (you can use about d8=400-500ms for small molecules and d8=100-200ms for large biomolecules)

A minimum two-scan cycle is needed (ns=2 and ds=2).

document.write(acqu);

The recorded data is Fourier transformed with ef (set lb=1) and automatic phase and baseline corrections are performed using apk and abs, respectively. Modify processing parameters if required from the ProcPars section or using the ased command.

Use the TOPSPIN plot editor (xwinplot)

It can be advisable to store all acquisition and processing parameters (with the command wpar) to be used later.

The best option is to use the shorter selective 180º pulse that achieve clean excitation of the selected resonance without disturb other resonances. In some cases, the use of long selective pulses can undergo important sensitivity losses due to relaxation effects.

The selective ge-1D NOESY experiment can also be recorded using a DPFGE scheme as a  starting building block (see DPFGE experiment for more details). NOE measurements can also be performed using the selective 1D ROESY analog. See details how to build-up a NOE curve as a function of the mixing time.