Saccharide NMR
13C relaxation data
13C data relaxation has been used to study molecular flexibility in polysaccharides. Using the model-free approach, a generalized order parameter (S2) is obtained, ranging from 1 for a completely rigid molecule to 0 if no preferred orientation is observed. Also correlations to the global correlation times and effective local correlation times for internal motions can be extacted. Examples have been reported ( 98JML255 ):
  • Mono- and disaccharides ( 87MRC521 , 89JMR530-85 , 96JPC17103 and 99MRC586 ). As an example, sucrose in a viscous cryosolvent, D2O-DMSO ( 00MRC1012 ) and a temperature and concentration-dependent relaxation study ( 02JPCB6331 ).
  • Trisaccharides ( 95MRC541 , 98JPCB1013 , and 02JPCB5275 )
  • Tetrasaccharides ( 92MRC733 )
  • Pentasaccharides ( 96JB1-7 and 99JACS11847 )
  • Nanosaccharides ( 97CRS219 )
  • Cyclodextrins ( 94JPC28-98 )
  • Homopolysaccharides ( 99CR16 ).
  • Other polysaccharides ( 05JB311-31 ).

    • The following NMR restraints are usually measured at multiple fields:

    T1 13C relaxation rates
    T2 13C relaxation rates
    13C{1H} heteronuclear NOEs
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