Location of Glycosidic Linkages: In traditional approaches, glycosyl linkage positions are determined by chemical derivatization (O-methylation) and GC-MS analysis. Several NMR approaches:

• Interresidue NOEs (that can not be always detected).
• ¹H-¹³C Coupling constants (³J_COCH) ( 93BIO12715 , 97JACS8695 , 99JACS9843 , 98MRC839 , 01MRC381 ).
• Four-bond ¹H-¹H coupling constants (when possible, from long-range COSY experiments).
• Study of OH hydroxyl protons at low-temperature in H2O and D2O ( 98AB63 )
• ¹³C-¹³C coupling constants (²J_COC and ³J_COCC) ( 96JMRB69-112 , 98JACS11158 , 99JACS9843 , 00JACS396 )
• Dipole-dipole cross-correlation using a 2D CT-HSQC experiment ( 00JACS8307 )