In oligosaccharides, the assignment of NMR resonances and the determination of the solution structure is a difficult task due to:

Small dispersion of chemical shifts in the 3.3-4.3 region

Limited number of NOEs when working in D2O

Sometimes, 13C-labeling is required

Presence of several conformations

Specific topics of interest:

Anomeric Region

Strong downfield Efefcts of O-acetylation.

Chemical shift of hydroxy protons as conformational probes

1H and 13C NMR Chemical shift data on

2-O, 3-O, and 2,3-Di-O-glycosilated methyl alpha- and beta-glycopiranosides and beta-galactopiranosides ( 93MRC599 )

Effects of Glycosylation on 13C chemical shift ( 88MRC735 , 90CSR29 )

13C Chemical shifts for the anomeric carbons. Dependence with glycosidic bond dihedral angles. ( 01JB49-21 )