Membrane Proteins

General Reading: 01JB243-19 , B99KRI121, 00ENC1281 , 98COP640 , B03ZER95 and 04CR3587 .

Topics:

  • Solid-State NMR methodology for membrane protein structure determination ( 00COP593 , 02CONC212 and 03CONC111 )
  • Micellar systems as solvents in Protein NMR ( 01METH271 )
  • NMR of membrane associated peptides and proteins ( 01METH285 , 03JB125-25 and 03CONC130 )
  • NMR experiments for labeled membrane proteins in oriented lipid bilayers ( 02JB225-22 , 03CONC130 and 05METH350 )
  • NMR of peptide in isotropic phospholipid bicellar solution ( 02JB103-24 )
  • NMR of bicelles ( 03JB113-25 )
  • Membrane protein preparation for NMR screening ( 05METH321 )
  • Solution structure and dynamics of membrane proteins by NMR ( 05METH335 )
  • Applications:

  • Myristoylation for inmobilization and alignment of soluble proteins. Measurement of orientation-dependent chemical shifts and residual dipolar couplings ( 03JB55-25 )
  • Structure and dynamics of a membrane protein in micelles from ( 03JB327-26 and 03JB377-27 ).
  • Backbone amide resonance assignments from 2D HMQC-NOESY spectra
  • Backbone dynamics from 1H-15N heteronuclear NOEs
  • Structure and topology of the helices from residual dipolar coupling constants
  • Evaluation of detergents for structural studies of membrane proteins ( 04JB43-28 )
  • Probing membrane protein orientation and structure using fast MAS ( 04JB253-30 )
  • Backbone and sidechain assignments in a transmembrane protein complex ( 05JB279-31 )
  • 99PNAS487 and 99BIO1562
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