Types of measurables RDCs

Typically, several types of residual dipolar couplings (RDCs) are measured in proteins:
  • One-bond coupling constants: 1DNH, 1DCA-HA, 1DCA-CB, 1DCA-CO, and 1DCO-N.
    Such RDC's are simple to analyze and measure because the internuclear distance is known and the magnitude of such couplings is relatively large.
  • Two-bond coupling constants: 2DHN-CO, 2DN-HA
  • These are the expected dipolar couplings when a maximum value of 25 Hz isfound for 1D(NH):