Empirical 3JHN-CB values and angular backbone constraints can be interconverted following well-known Karplus relationships: 99JB263 .VALUES
3JHN-CB=2.78*cos2(fi+60) - 0.37*cos(fi+60) + 0.03 ( 96JACS2483 ) 3JHN-CB=2.90*cos2(fi+60) - 0.56*cos(fi+60) + 0.18 ( 99JB1-14 ) where fi is the backbone torsion angle.
Range from -0.2 Hz to +3.2 HzMEASUREMENTThe expected 3JHN-CB coupling constant values found in the secondary structure of proteins:
- Alpha-helix: 2.8 Hz
- 310-helix: 2.5 Hz
- Beta-strand: 0.8 Hz
RELATED TOPICS
- 3D HNCA[CB]-E.COSY experiment.
- 3D 1H-15N NOESY-HMQC and 4D 13C-HMQC-NOESY-15N-HMQC ( 94JMRB172-104 )
- 3D (H)CANNH[CB]-E.COSY experiment ( 95JB25 )
- 3D heteronuclear relayed E.COSY experiment combined with 2D multiplet line-shape analysis ( 95JB95 ).
- 3D HNCACB[NH] -E.COSY experiment ( 99JB263 ).
- 3D HNHB[CB]-E.COSY experiment ( 99JB263 ).
The same dihedral angle information can be obtained from other coupling constants values:
