The 2D (HB)CB(CGCD)HD experiment allows to correlate the side-chain CB chemical shift with the HD aromatic protons in ¹³C-labeled proteins in D₂O.

Implementation on any AVANCE spectrometer equipped with an inverse probehead, a third channel and pulsed field gradients (PFGs).

The original sequence was reported in 93JACS11054 and a 3D (HB)CB(CG)CDHD experiment has been also proposed ( 96JMRB259-112). Related experiments:

Analog 3D version but using the reverse pathway (3D H(CD)CGCB experiment) ( 96JMRB259-112 ). A similar 3D HCD(CG)CB experiment has been proposed (02JB153-164) for sequence-specific histidine residues.

3D version including a second ¹³C-¹³C COSY step (3D (HB)CB(CGCDCE)HE experiment) ( 93JACS11054 ).

Analog 2D version including ¹³C-¹³C isotropic mixing (2D (HB)CB(C_aro)H experiment) ( 96JB161 ).

See other NMR methodologies for aromatic resonance assignment