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DESCRIPTION
The 2D Arg-He(NeCz)Nh experiment allows the sequence-specific assignment
of arginine He guanidino protons and Nh nitrogens in 13C,15N-labeled proteins
in H2O.
REQUIREMENTS
Implementation on any AVANCE spectrometer equipped with an
inverse probehead, a third channel and pulsed field gradients (PFGs).
VERSIONS
The original sequence was reported in
94BIO14858
,
95JACS3556
.
EXPERIMENTAL DETAILS
SPECTRA
A 2D 1H-15N corerlation map is obtained correlating
He guanidino protons
(in F2) and Nh nitrogens (in F1) of arginine residues.
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