Implementation on any AVANCE spectrometer equipped with an inverse probehead, a third channel and pulsed field gradients (PFGs). The experiment is better applied on ¹³C-labeled proteins in D₂O.

The original sequence was reported in 95JACS6527 using the AMNESIA scheme. An analog 2D version (2D (HB)CB(C_aro)H experiment) has been also published using PLUSH-TACSY ( 96JB161 ). See other NMR methodologies for aromatic resonance assignment