2H-decoupling in triple-resonance experiments

For ²H-labeled proteins, Deuteron decoupling (usually using WALTZ-16) in the aliphatic region is usually applied during the ¹³C-evolution time t₁ when the magnetization is on the alpha carbons. The broadband ¹H-decoupling is usually interrupted during this time period and the corresponding proton-carbon couplings are refocused by a 180 degree ¹H-pulse, instead.

In the example we can observe a standard building block in which ¹³CA evolves during the constant 2*d5 period and the heteronuclear J_NCA and J_CACO couplings do not evolve during the CT period.

The major modifications to be included, with respect to the original experiments are:

• The nucleus on f4 channel is defined as NUC4=2H
• o4p=3.0ppm, centered to the aliphatic region.
• pl4=120, power level for hard pulses on f2 channel
• Deuteron decoupling is achieved by applying cpdgrp4=waltz16, at power level pl17, using a decoupling pulse length about pcpd4=300us.

A number of parameter set and pulse programs are available.