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For 13C,15N-labeled proteins, in some cases it is necessary to use selective 13C-decoupling, for instance on the carbonyls or alpha-carbons.REQUIREMENTS
EXPERIMENTAL DETAILS
SYNTAX
Set the following parameters:RELATED TOPICS
- cpdgrp2=mlev180sp
- power level sp15=pl12
- decoupling pulse length about pcpd2=768us
- spnam15=G3.256
- spoff15 is the 13C-offset for selective decoupling.