INEPT block using SPIN-LOCK
DESCRIPTION
Sometimes, a purgued spin-lock period of 1-2 ms of duration is incorporate in the initial INEPT block of the HSQC experiment.
REQUIREMENTS
Easy implementation on AVANCE spectrometers.
EXPERIMENTAL DETAILS
SYNTAX
The standard way to implement the spin-lock period into the INEPT block in a pulse program should be:
...
(p1 ph1)
d4
(p2 ph1) (p4 ph3):f2
d4
p28 ph1
d4
(p1 ph2) (p3 ph4):f2
...
in which p28 is the trim pulse of 1-2 ms applied at high-power level.

RELATED TOPICS
The INEPT block is used as a preparation period of many NMR experiments as, for instance, the 2D HSQC experiment which is the basis of many 3D and 4D triple-resonance experiments specifically designed for labeled biomolecules.
ml");