In many 3D and 4D NMR experiments for
13C,15N-labeled proteins,
it is necessary to decouple 1H during
15N and/or 13C evolution periods. Such decoupling
(usually performed with DIPSI-2)
prevents transverse relaxation, giving further gain in sensitivity
compared to the use of 180 degree pulses. For reproducible
water suppression it is essential to perform the decoupling in a synchronous
mode.
This is usually performed by applying a DIPSI-2 decoupling scheme embedded by 90 1H pulses applied
from an orthogonal axis.