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1H-decoupling in 3D experiments

DESCRIPTION
In many 3D and 4D NMR experiments for 13C,15N-labeled proteins, it is necessary to decouple 1H during 15N and/or 13C evolution periods. Such decoupling (usually performed with DIPSI-2) prevents transverse relaxation, giving further gain in sensitivity compared to the use of 180 degree pulses. For reproducible water suppression it is essential to perform the decoupling in a synchronous mode.

This is usually performed by applying a DIPSI-2 decoupling scheme embedded by 90 1H pulses applied from an orthogonal axis.

REQUIREMENTS
EXPERIMENTAL DETAILS
Low-power 90º 1H pulse for broadband DIPSI-2 decoupling via the observe f1 channel (pcpd1 at pl19 using cpdprg1=dipsi2).
SYNTAX
The standard way to implement 1H decoupling in a 3D pulse program is:
...
d12 pl19:f1
(p26 ph2):f1
d12 cpds1:f1 ph1
...
d12 do:f1
(p26 ph3):f1
...
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