Broadband-decoupling in 3D experiments

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For ¹³C,¹⁵N-labeled proteins, you should implement broadband X-decoupling during the Y-evolution and during the acquisition, in order to prevent the splitting of the alpha-proton signal due to coupling to the bound carbon. You can achieve this by inserting a 180 degree X pulse in the middle of the Y-evolution, optionally centered with respect to the 180 degree pulse for other nucleus.

A standard way to implement broadband X decoupling during Y evolution in a pulse program should be:

... CEN_HC2=(p22-p4)/2" ... d0 pl2:f2 (p22 ph0):f3 (CEN_HC2 p4 ph0):f2 d0