Adiabatic decoupling in 3D experiments

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For ¹³C,¹⁵N-labeled proteins, You might want to use the adiabatic ¹³C-decoupling while it enables lower power, and thus, less heating. An approriate power level is +2dB with respect to the power level determined for ¹³C-GARP-decoupling. You can create the shaped pulse CHIRP95 optimized for minimal sidebands, with the 'shape tool', stdisp.

• Select the shape smoothed chirp
• change the total sweep width to 60 000 MHz and the length of pulse to 1500us.
• Save it under the name CHIRP95.

Set the following parameters:

• CPDPRG2=p5m4sp180 is the decoupling program
• PCPD2=1.5m is the decoupling pulse length
• PL12 is the decoupling power level
• SP15=pl12 (note: + 2dB compared to garp)
• SPNAM15=CHIRP95 is the adiabatic shaped pulse