Implementation on AVANCE spectrometers equipped with a triple-resonance probehead is feasible. Improved versions using pulsed field gradients (PFGs) are also available and, therefore, in such cases gradient technology is required. The experiment is recorded on a ¹³C,¹⁵N-labeled protein in H₂O.

The 4D HN(CA)NH sequence was described in 97JMR214-124 (DEU)

The 4D HN(CA)NH experiment can be recorded in automation mode.