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4D HNCAHA

DESCRIPTION
The 4D HNCAHA experiment has been designed to trace out many intraresidue and sequential backbone connectivites in large proteins using a single experiment. The same connectivities can be also observed in a 4D HACANH experiment in which the coherence transfer is reversed. Sequential correlations can be unambiguosly identified ina closely related 4D 4D HN(CO)CAHA or HACA(CO)NH experiments

REQUIREMENTS
Implementation on AVANCE spectrometers equipped with a triple-resonance probehead is feasible. Improved versions using pulsed field gradients (PFGs) are also available and, therefore, in such cases gradient technology is required. The experiment is recorded on a 13C, 15N-labeled protein in H2O.
VERSIONS
The 4D HNCAHA sequence was described in 92JMR443-98 , 92JB631 , 92JMR444-100 and 94JMRB193-104 .

Related 4D HACANH (or HCANNH) experiment has been also reported ( 92JB631 , 92JACS2262 and 93JMRB223-101 ).

The 4D pulse sequence is a simple extension of the 3D HA(CA)NH and 3D HN(CA)HA experiments and related sequences like 3D (HA)CANH, HACA(N)H, 3D H(N)CAHA, and 3D (H)NCAHA experiments.

Closely related 4D [HAHB][CACB]NH ( 96JACS407 ) and 4D HCCNH pulse sequences have been also published . .

EXPERIMENTAL DETAILS
The 4D HNCAHA experiment can be recorded in automation mode.
SPECTRA
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