Implementation on AVANCE spectrometers equipped with a triple-resonance probehead is feasible. Improved versions using pulsed field gradients (PFGs) are also available and, therefore, in such cases gradient technology is required. The experiment is recorded on a ¹³C,¹⁵N-labeled protein in H₂O.

The 4D HCC(CO)NNH sequence was described in 93JB349 96JACS407&

4D HC(CO)NH-TOCSY 
92FEBS413

The 4D HCC(CO)NNH experiment can be recorded in automation mode.