Implementation on AVANCE spectrometers equipped with a third channel. Improved versions using pulsed field gradients (PFGs) are also available and, therefore, in such cases gradient technology is required.

The 3D H(N)CACO experiment is applied on ¹³C,¹⁵N-labeled proteins in H₂O.

The original H(N)CACO experiment was proposed in 93JB233 .

Related Experiments:

3D HN(CA)CO experiment

See list of 3D triple-resonance NMR experiments for doubly-labeled proteins.