The 3D CBCA(CO)NH experiment is specifically designed to correlate the ¹⁵N amide resonance of one residue with ¹HA, ¹HB , ¹³CA and ¹³CB resonances of its preceding residue via the intervening ¹³CO spin by means of the ¹J(N,CO), ¹J(CA,CO), ¹J(CH) and ¹J(CA,CB) coupling constants.

Implementation on AVANCE spectrometers equipped with a third channel. Improved versions using pulsed field gradients (PFGs) are also available and, therefore, in such cases gradient technology is required.

The experiment is applied on ¹⁵N,¹³C-labeled proteinsin D₂O.

More details on practical implementation of the 3D CBCA(CO)NH experiment on AVANCE spectrometers can be found in the corresponding tutorial.

See list of 3D triple-resonance NMR experiments for doubly-labeled proteins.