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3D HMQC-TOCSY

DESCRIPTION
The 3D HMQC-TOCSY experiment is specifically designed to obtain X-edited TOCSY spectra from which 1H-1H TOCSY correlations can be clearly assigned even in overcrowded regions (the mechanism involves an heteronuclear transfer via 1J(XH) followed by a TOCSY step). Similar results can be obtained from an analog 3D HSQC-TOCSY experiment.
 
 
REQUIREMENTS
Implementation on AVANCE spectrometers is feasible. Improved versions using pulsed field gradients (PFGs) are also available and, therefore, gradient technology is required. The 1H-13C HMQC-TOCSY experiment is usually applied on 13C-labeled proteins in D2O and at natural abundance on concentrated samples. On the other hand, the 1H-15N HMQC-TOCSY is usually applied on 15N-labeled molecules in H2O.
VERSIONS
The original 3D HMQC-TOCSY pulse sequence ( 89JMR634-84 and 92JMR433-99 ) basically consists of a 2D 1H-X HMQC pulse sequence followed by an homonuclear 2D TOCSY pulse sequence. Related experiments are: Several improved versions have been proposed incorporating the following modifications:
EXPERIMENTAL DETAILS
The 3D HMQC-TOCSY experiment can be recorded in automation mode. Both blocks are usually optimized as in their 2D counterparts. More details on practical implementation of the 3D HMQC-TOCSY  experiment on AVANCE spectrometers can be found in the corresponding Tutorial 3D HMQC-TOCSY experiment
SPECTRA
The HMQC-TOCSY experiment affords a 3D spectrum in which X, 1H and 1H chemical shifts are displayed in three independent dimensions. Thus, the F2(1H)-F3(1H) plane of a 1H-15N HMQC-TOCSY spectrum will show a typical 2D F1(NH)-F2(1H) TOCSY map. On the other hand, the F2(1H)-F3(1H) plane of a 1H-13C HMQC-TOCSY spectrum will show a typical 2D F1(1H aliphatic)-F2(1H) TOCSY map while the F1(13C)-F2(1H) plane will correspond to a conventional 2D 1H-13C HMQC spectrum.
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