The 2D w₁,w₂-13C/15N-doubly filtered NOESY experiment affords a 2D NOESY spectrum in which 1H directly-attached to both 13C/15N nuclei are efficiently filtered before and after evolution during the t1 and the acquisition periods. This experiment is widely used to study protein-ligand complexes, where the protein is usually ¹³C and/or ¹⁵N-labeled and the ligand is at natural abundance .

Easy implementation on any AVANCE spectrometer equipped with an inverse probehead.

13C/15N half filters ( 92JACS2433 , 96JB492-8 , 99JB43 ). In such cases, several options are feasible considering, for instance, ¹³C/¹²C isotopes:

• ¹³C(w1)-¹³C(w2) doubly filtered
• ¹³C(w1)-filtered/¹³C(w2)-selected
• ¹³C(w2)-filtered/¹³C(w1)-selected
• ¹³C(w1)-¹³C(w2) doubly selected

Other closely related experiments are:

• 2D Inverse experiments
• 2D Inverse gradient-enhanced experiments
• 2D w₁-X-edited NOESY experiment.
• 2D w₂-X-edited NOESY experiment.
• 3D HMQC-NOESY-HMQC experiment
• 4D HMQC-NOESY-HMQC experiment