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See Homodecoupling experimentREQUIREMENTS
Easy implementation on AVANCE spectrometers.VERSIONS
In a band-selective homodecoupling experiment, a conventional 1H spectrum is recorded. From the second channel, specific region-selection irradiation is applied on a predefined frequency during the acquisition period (see homodecoupling building block).EXPERIMENTAL DETAILS
The homodecoupling experiment can be acquired, processed and plotted in a fully automated way. Minor changes from a predefined parameter set are required. Important parameters to consider are the offset and the power level of the irradiation. It is useful to have a calibration of the field strength delivered from the decoupler in order to optimize the required selectivity and to minimize Bloch-Siegert shift effects.SPECTRAFor further details on practical implementation on AVANCE spectrometers see Tutorial: Homodecoupling
The homodecoupling experiment affords a conventional 1H spectrum in which the irradiated signal is missing and all protons J-coupled to this irradiated 1H will show residual multiplet structure (the corresponding J coupling splitting has also been removed). Comparison between multiplet structure of the conventional 1H spectrum and the homodecoupling spectrum allows to measure and to assign coupling constants values.RELATED TOPICS![]()
Also see Homodecoupling Test
A 2D COSY experiment can be a more useful alternative to trace out all J-connectivities from a single spectrum.