General Strategies for Structure Elucidation by NMR

Example III.
2mM lysozyme dissolved in 90% H20/10% D20.
Inverse broadband probehead incorporating Z-gradients in an AVANCE 500 MHz spectrometer.

Main Objective: Complete and inambiguous assignment of 1H spectrum.

  1. 1H spectrum using presaturation or WATERGATE.
  2. 2D TOCSY experiments using several mixing times.
  3. 2D NOESY experiments using several mixing times.
Notes. 2D TOCSY experiment is also recorded in D20.