General Strategies for Structure Elucidation
by NMR
Example III.
2mM lysozyme dissolved in 90% H20/10%
D20.
Inverse broadband probehead incorporating Z-gradients in an AVANCE
500 MHz spectrometer.
Main Objective: Complete and inambiguous assignment of 1H
spectrum.
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1H spectrum using presaturation or WATERGATE.
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2D TOCSY experiments using several mixing times.
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2D NOESY experiments using several mixing times.
Notes. 2D TOCSY experiment is also recorded in D20.