• Record a conventional 1H-decoupled 13C spectrum.

a DEPT-135 spectrum with rpar C13DEPT135 all (the pulse program dept135

See  90º 13C observe pulse and 90º 1H decouple pulse calibrations.

By default, the following parameters are set to: ns=128, ds=2, d1=2, d2=3.57ms, sw=331.2 and td=64k. Change them if required.

Optimize the values of o1 and sw from the conventional 13C spectrum if required. o2 must be set to the center of the proton spectrum.

(au_zg135)) (proc_cpd135).

. In automation applications the signal-to-noise ratio can be automatically optimized changing the acquisition AU program to AUNM=au_deptsino.

For instance, to record a DEPT-90 spectrum, read the predefined parameter set of DEPT-135 experiment (rpar dept45 all), change the pulse program (pulprog=dept90) and start the acquisition (check for the number of scans, ns). These parameters can be stored for further use (wpar dept90 all). Process the spectrum as described above.

The same procedure should be followed to record a DEPT-45 spectrum: read the predefined parameter set of DEPT-135 experiment (rpar dept135 all), change the pulse program (pulprog=dept45) and start the acquisition (check for the number of scans, ns). These parameters can be stored for further use (wpar dept45 all).

Otherwhise, a single parameter set can be defined (wpar dept all) for recording any DEPT experiment. In such case, the pulse program is set to dept and the user must define the corresponding p0 (45, 90 or 135 degree ¹³C observe pulse) according to the carbon multiplicity selection.

    1D 1H-decoupled carbon-13 spectrum